A Modified Perfusion Method to Improve the Quality of Procured Donor Pancreas in Rats

Fu Tian Du, Hong Feng Lin, Wei Ding

Abstract


Background: In this animal study, we evaluated a modified pancreas perfusion method to improve the quality of harvested pancreas in rats. In this model, the portal vein was used as the outflow route during the pancreas perfusion.

Methods: Forty-eight male Wistar rats were randomly divided into study group and control group, with 24 rats in each group. In the study group, the portal vein was used as outflow of perfusion. While in the control group, the post-hepatic vein (right artrium) was used as perfusion outflow. UW solution was used as perfusion and preservation solution. Pancreas tissue samples were collected at 6, 10, and 14 hours after perfusion and cold preserved for histology and immunohistochemistry examination, P-selection (PS) and ICAM-1 were determined. Pancreas samples were also examined using electronic microscope for ultra-structures.

Results: Compared with the study group, in the pancreas of control group there were significant pathological impairments and cellular ultra-structural alterations observed by immunohistochemistry and electronic microscope, and these impairments aggravated with time. There were mild histological alterations in the pancreas of study group.

Conclusions: During the donor pancreas perfusion, the early opening of portal vein as the outflow is better than the opening of the post-hepatic vein for the preservation of donor graft pancreas and the reduction of tissue impairments.




doi: http://dx.doi.org/10.4021/gr501e


Keywords


Pancreas transplantation; Donor graft; P-selectin; Intercellular adhesion molecule 1

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