Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access
Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc
Journal website http://www.gastrores.org

Original Article

Volume 2, Number 2, April 2009, pages 110-114

Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma

Figures

Figure 1.
Figure 1. Marker: DL2000, 1% agarose ORF of ES274070 semi-quantitative RT-PCR. 1, 2……9: sample number; N: normal colorectal tissues; T: colorectal adenocarcinoma tissues.
Figure 2.
Figure 2. The result of ES274071 ORF sequencing.
Figure 3.
Figure 3. Marker: DL2000, 1% agarose ORF of ES274076 semi-quantitative RT-PCR. 1, 2……9: sample number; N: normal colorectal tissues; T: colorectal adenocarcinoma tissues.

Tables

Table 1. PCR conditions
 
Accession numberPCR condition
ES27407094°C 3 min; 94 °C 30 sec, 54 °C 30 sec, 72 °C 1 min, 30 cycles; 72 °C 5min
ES27407194 °C 4 min; 94 °C 30 sec, 52.5 °C 30 sec, 72 °C 2 min, 30 cycles;72 °C 7min
ES27407694 °C 4 min; 94 °C 30 sec, 52 °C 30 sec, 72 °C 2 min, 33 cycles; 72 °C 7min
ES27407394 °C 4 min; 94 °C 30 sec, 57 °C 30 sec, 72 °C 2 min, 33 cycles; 72 °C 7min

 

Table 2. PCR primers and Products
 
Accession numberPrimerProduct
ES274070Forward 5’ –TTGGAGCCCTGAGTATCTGTG -3’
Reverse 5’ –TAATGGAACCTGGTGCTAAGTC -3’		616bp
ES274071Forward 5’-CCTTCGCCTTCCCTTCTC-3’
Reverse 5’-CGACTGAGCACAAGAGGGA-3’		700bp
ES274076Forward 5’ –GCACTGCCAAGATAGACAA -3’
Reverse 5’ –CTGGAACCTGCTACGAAT -3’		512bp
ES274073Forward 5’ –TACCACCTACCTCCCTCA -3’
Reverse 5’ –ATGGGCTGGGTTTTACTA -3’		791bp