Gastroenterol Res

Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access

Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc

Journal website http://www.gastrores.org

Original Article

Volume 1, Number 1, December 2008, pages 33-39

Inhibition of de novo Methyltransferase 3B is a Potential Therapy for Hepatocellular Carcinoma

Figure 1. Hierarchical clustering based on gene expression data from SMMC-7721 DNMT3B unsuppressed and suppressed cells, and SMMC-7721 5-Aza-CdR untreated and treated cells.

**Table 1.** Genes altered more than 2-fold after 5-Aza-CdR and pMT1 treatment in the hepatocellular carcinoma SMMC-7721 cell line.
	GenBank_ID	Gene_Symbol	5’CpG island (CGI)a	Function
^aBased on a PubMed search; CGI, 5’ CpG island; None, no known 5’ CpG island.
Regulation of transcription	NM_182917	EIF4G1	CGI	regulation of translational initiation

Signal transduction	NM_002733	PRKAG1	CGI	regulates adipose differentiation
	NM_004583	RAB5C	CGI	signal transduction, regulation

Others	NM_016219	MAN1B1	CGI	hydrolase activity
	NM_017883	WDR13	CGI	mRNA processing
	NM_000526	KRT14	None	epidermis development
	NM_004860	FXR2	CGI	insulin receptor binding
	NM_005993	TBCD	CGI	integrin-mediated signaling pathway
	NM_000034	ALDOA	CGI	RNA binding

Table 1. Genes altered more than 2-fold after 5-Aza-CdR and pMT1 treatment in the hepatocellular carcinoma SMMC-7721 cell line.

GenBank_ID

Gene_Symbol

5’CpG island (CGI)a

Function

^aBased on a PubMed search; CGI, 5’ CpG island; None, no known 5’ CpG island.

Regulation of transcription

NM_182917

EIF4G1

CGI

regulation of translational initiation

Signal transduction

NM_002733

PRKAG1

CGI

regulates adipose differentiation

NM_004583

RAB5C

CGI

signal transduction, regulation

Others

NM_016219

MAN1B1

CGI

hydrolase activity

NM_017883

WDR13

CGI

mRNA processing

NM_000526

KRT14

None

epidermis development

NM_004860

FXR2

CGI

insulin receptor binding

NM_005993

TBCD

CGI

integrin-mediated signaling pathway

NM_000034

ALDOA

CGI

RNA binding

**Table 2.** Genes altered by more than 2.5-fold after pMT3B and 5-Aza-CdR treatment in the hepatocellular carcinoma cell line SMMC-7721
GenBank_ID	Symbol	5 CpG island (CGI)a	Function
^aBased on a PubMed search; CGI, 5’ CpG island; None, no known 5’ CpG island.
Cell differentiation
NM_006096	NDRG1	CGI	cell differentiation

Cell proliferation
NM_005557	KRT16	NO	cell proliferation
NM_013402	FADS1	CGI	regulation of cell differentiation

Regulation of transcription
NM_003926	MBD3	CGI	regulation of transcription
NM_005253	FOSL2	CGI	regulation of transcription

Signal transduction
NM_138769	ARHT2	CGI	signal transduction
NM_004218	RAB11B	CGI	signal transduction
NM_145173	DIRAS1	CGI	signal transduction

Others
NM_017510	HSGP25L2G	CGI	intracellular protein transport
NM_006700	FLN29	CGI	nucleic acid binding
NM_001956	EDN2	NO	protein kinase C activation
NM_057089	AP1S1	CGI	intracellular protein transport
NM_020196	XAB2	CGI	DNA repair
NM_006412	AGPAT2	CGI	metabolism
NM_001074	UGT2B7	NO	metabolism
NM_006247	PPP5C	CGI	regulation of I-kappaB kinase/NF-kappaB cascade
NM_002273	KRT8	NO	cytoskeleton organization and biogenesis
NM_006445	PRPF8	CGI	nuclear mRNA splicing, via spliceosome
NM_002233	KCNA4	CGI	potassium ion transport
NM_004204	PIGQ	CGI	carbohydrate metabolism
NM_004818	DDX23	CGI	protein kinase C activation
NM_017458	MVP	NO	response to drug
NM_032038	SPINL	CGI	tetracycline transport
NM_020230	PPAN	CGI	RNA splicing
NM_001897	CSPG4	CGI	cell motility
NM_021979	HSPA2	NO	male meiosis
NM_018110	DOK4	CGI	insulin receptor binding
NM_144568	C14orf9	CGI	hydrolase activity

Table 2. Genes altered by more than 2.5-fold after pMT3B and 5-Aza-CdR treatment in the hepatocellular carcinoma cell line SMMC-7721

GenBank_ID

Symbol

5 CpG island (CGI)a

Function

^aBased on a PubMed search; CGI, 5’ CpG island; None, no known 5’ CpG island.

Cell differentiation

NM_006096

NDRG1

CGI

cell differentiation

Cell proliferation

NM_005557

KRT16

cell proliferation

NM_013402

FADS1

CGI

regulation of cell differentiation

Regulation of transcription

NM_003926

MBD3

CGI

regulation of transcription

NM_005253

FOSL2

CGI

regulation of transcription

Signal transduction

NM_138769

ARHT2

CGI

signal transduction

NM_004218

RAB11B

CGI

signal transduction

NM_145173

DIRAS1

CGI

signal transduction

Others

NM_017510

HSGP25L2G

CGI

intracellular protein transport

NM_006700

FLN29

CGI

nucleic acid binding

NM_001956

EDN2

protein kinase C activation

NM_057089

AP1S1

CGI

intracellular protein transport

NM_020196

XAB2

CGI

DNA repair

NM_006412

AGPAT2

CGI

metabolism

NM_001074

UGT2B7

metabolism

NM_006247

PPP5C

CGI

regulation of I-kappaB kinase/NF-kappaB cascade

NM_002273

KRT8

cytoskeleton organization and biogenesis

NM_006445

PRPF8

CGI

nuclear mRNA splicing, via spliceosome

NM_002233

KCNA4

CGI

potassium ion transport

NM_004204

PIGQ

CGI

carbohydrate metabolism

NM_004818

DDX23

CGI

protein kinase C activation

NM_017458

MVP

response to drug

NM_032038

SPINL

CGI

tetracycline transport

NM_020230

PPAN

CGI

RNA splicing

NM_001897

CSPG4

CGI

cell motility

NM_021979

HSPA2

male meiosis

NM_018110

DOK4

CGI

insulin receptor binding

NM_144568

C14orf9

CGI

hydrolase activity